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Mating system and species concept in heterothallic species of Aspergillus section Fumigati
Dudová, Zuzana ; Hubka, Vít (advisor) ; Mencl, Karel (referee)
The aim of this study was to determine the species boundaries within the Aspergillus viridinutans species complex. The species belonging to this complex are predominantly soil- inhabiting organisms that are increasingly reported as opportunistic human and animal pathogens. A total number of 98 isolates from various substrates and countries was subjected to morphological, physiological and molecular analysis (calmodulin, β-tubulin, actin and RNA polymerase II subunit 2 gene) and mating experiments were provided on different media and temperatures. Some other heterothallic species from section Fumigati with unknown sexual state were analysed using similar method as well. Key words: Aspergillus viridinutans, Aspergillus turcosus, phylogenetic analysis, mating- type genes, anamorph, teleomorph, mating experiments, MAT1-1, MAT1-2
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Induction of sporulation and sexual state formation in Ascomycota (Fungi)
Dudová, Zuzana ; Kolařík, Miroslav (advisor) ; Novotný, David (referee)
The aim of this study was to summarize factors that induce sporulation of anamorph and fruiting body formation in ascomycetous fungi. These factors are, depending on a species, light, water, air and nutrients availability, presence of various chemical compounds and osmolarity. Specific media and cultivation conditions used for sporulation induction are reviewed. I concentrated on mating type (MAT) genes and genes involved in the process of conidiation in the chapter about genetic background of fructification and sporulation. Key words: sporulation, fruiting body formation, MAT genes, mating factors, anamorph, teleomorph, Ascomycota, homothallism, heterothallism.
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